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Rapid Micro-propagation and Establishment of Leaf Regeneration System of Poplar 741

Xue Yu

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Abstract

Under the conditions of different concentrations of 6BA and NAA,we employed such explants as stem segments and plantlet leaves of poplar 741 to investigate its micro-propagation and leaf regeneration system.Results indicate that the optimal medium for bud multiplication is MS+6-BA0.5 mg/L+NAA 0.1 mg/L;the best rooting medium is MS+IBA 0.3 mg/L;the appropriate medium for shoot regeneration of a leaf is MS+6-BA 1.0 mg/L+NAA 0.1 mg/L.The experiment also studied the position effects of different leaves and the effects of placement of a leaf in media.

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What this paper is about

Under the conditions of different concentrations of 6BA and NAA,we employed such explants as stem segments and plantlet leaves of poplar 741 to investigate its micro-propagation and leaf regeneration system.Results indicate that the optimal medium for bud multiplication is MS+6-BA0.5 mg/L+NAA 0.1 mg/L;the best rooting medium is MS+IBA 0.3 mg/L;the appropriate medium for shoot regeneration of a leaf is MS+6-BA 1.0 mg/L+NAA 0.1 mg/L.The experiment also studied the position effects of different leaves and the effects of placement of a leaf in media.

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Available abstract

Under the conditions of different concentrations of 6BA and NAA,we employed such explants as stem segments and plantlet leaves of poplar 741 to investigate its micro-propagation and leaf regeneration system.Results indicate that the optimal medium for bud multiplication is MS+6-BA0.5 mg/L+NAA 0.1 mg/L;the best rooting medium is MS+IBA 0.3 mg/L;the appropriate medium for shoot regeneration of a leaf is MS+6-BA 1.0 mg/L+NAA 0.1 mg/L.The experiment also studied the position effects of different leaves and the effects of placement of a leaf in media.

Key concepts: Plantlet, Regeneration (biology), Shoot, Explant culture, Botany, Horticulture, Biology, Chemistry

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