Study on Fast Regeneration System for in Vitro Leaves of Poplar Reyang 1

[Objective] This study was to explore the rapid regeneration technique of in Vitro leaves in poplar Reyang 1.[Method] With poplar Reyang 1 as tested material,the effects of different plant growth regulators on inducing callus,sprouts and roots were investigated.[Result] Among 25 MS mediums added different combinations of plant growth regulators,the MS medium added with 6-BA 0.5 mg/L and NAA 0.05 mg/L had the highest rate of inducing callus,researching 100%.Among 36 mediums of inducing regenerative sprouts,the best was MS+6-BA 0.5 mg/L+NAA 0.05 mg/L+IBA 0.1 mg/L and it had 100% of inducing rate,with high quality sprouts.Too higher or lower concentration of 6-BA,NAA and IBA had no benefit for sprouting.Mixed application of IBA and NAA benefited for root generation,and the best medium for rooting was 1/2 MS+ NAA 0.5 mg/L+IBA 0.15 mg/L.The rooted seedlings could grow well after being transplanted in greenhouse.[Conclusion] With the leaves of poplar Reyang 1 as explants,the high-efficient regeneration system was established.