Establishment of Leaf-explant Regeneration System of Poplar 84K
Fan Jun, NW Sci
Abstract
Fan Jun, NW Sci
Abstract
Through the application of orthogonal designing, a systematic research into the establishment of the leaf explant regeneration systems for the poplar 84K was for the first time carried out. The optimal differentiation medium and proportion of hormone for leaf explant is MS+BA1.5 mg/L+NAA0.1 mg/L, and the optimal rooting medium and proportion of hormone for shoot is GMS+NAA0.01mg/L+IBA 0.25 mg/L. The results achieved laid a sound foundation for regeneration of poplar 84k on an industrial basis and gene transformation by means of leaf disc method.
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Through the application of orthogonal designing, a systematic research into the establishment of the leaf explant regeneration systems for the poplar 84K was for the first time carried out. The optimal differentiation medium and proportion of hormone for leaf explant is MS+BA1.5 mg/L+NAA0.1 mg/L, and the optimal rooting medium and proportion of hormone for shoot is GMS+NAA0.01mg/L+IBA 0.25 mg/L. The results achieved laid a sound foundation for regeneration of poplar 84k on an industrial basis and gene transformation by means of leaf disc method.
Key concepts: Explant culture, Regeneration (biology), Biology, Botany, Shoot, Transformation (genetics), Horticulture, Gene