2010•Northern HorticultureRequires access
Establishment of an Efficient Regeneration System for the Populusadenopoda×the poplar 84K
Zhou Yong-xue
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Abstract
Through the application of orthogonal designing,stems and leaves of Populusadenopoda×the poplar 84K were used as explants to study the optimal differentiation medium and proportion of hormone.The results showed that the optimal differentiation medium and proportion of hormone of stems and leaves were respectively MS+TDZ 0.0015 mg/L+NAA 0.05 mg/L and MS+TDZ 0.001 mg/L+NAA 0.01 mg/L,and1/2MS+NAA 0.01 mg/L+IBA 0.1 mg/L for adventitious root.Using the optimal regenertion system,regeneration rate of stems was 91%,regeneration rate of leaves was 70%,rooting frequency was 100%.In the study of rooting rate on carbon,the impact of sucrose was largest,on which the rate of rooting was 88.9%.The results achieved laid a sound foundation for regeneration of Populusadenopoda×poplar 84k on an industrial basis and gene transformation by means of leaf disc method.