Studies on physical and chemical properties and immunological activities of polysaccharides from Pleurotus ferulae

Crude polysaccharides A and B were extracted separately from the fruiting body and cultured mycelium of Pleurotus ferulae Lanzi with water-extracted method. For obtaining polysaccharide A1,protein was removed from crude polysaccharide A with Sevag method, dialysed with water, precipitated with CTAB and alcohol, dissolved with NaCl solution and then dialysed again . Identification of ultraviolet spectrum showed that the polysaccharide A1 was a homogeneous composition, and the purity of polysaccharide was 82.9% ,judging by the Phenol-H2SO4 method analysis. GPC (gel permeation chromatography)detection showed that the number average molecular weight of polysaccharide A1 was 141,088 and the weight average molecular weight of polysaccharide A1 was 142,897. GC (gas chromatography) analysis indicated the monosaccharides' composition and molar ratio of polysaccharide A1 were:Xyl∶Gla∶Glc=1∶1.102∶2.899.It was proved the crude polysaccharide A and B had immunological activities according to the experiments of macrophage phagocytosis, delayed type hypersensitivity and induction and detection of IL-2.