PURIFICATION,CONTENT DETERMINATION,PRELIMINARY COMPONENT ANALYSIS AND STRUCTURE IDENTIFICATION OF LYCD POLYSACCHARIDE

The polysaccharide of live yeast cell derivatives(LYCD)was studied.The crude polysaccharide was precipitated with 95 % ethanol in method of ethanol precipitation and the protein was removed by Sevege method.Then the content was determined by the phenol-sulfuric acid method.The crude polysaccharide was isolated and purified by Sephadex G-75.Then the components of the product was considered by thin-layer chromatography.Finally,the structure of polysaccharide were analyzed preliminarily by infra-red spectrum.The results showed that three times were best to remove the protein.Under this condition 8.21 mg/L polysaccharides were obtained.The LYCD polysaccharides had one kind of polysaccharide which contained D-glucose after hydrolysis.The results of IR spectrum showed that LYCD polysaccharides had the general structure of polysaccharides.