The Research of the Extraction,Purification,Structure Analysis of Pleurotus Ferulae Polysaccharide

The purpose of this study was to extract and purificate polysaccharide from the Pleurotus ferulae Lenzi,the basic physical and chemical properties of polysaccharide were obtained,and build a solid foundation at the further polysaccharide from the Pleurotus ferulae Lenzi exploration in the structure,relationship between structure and activity in theory.Pleurotus ferulae polysaccharide was prepared by ethanol precipitation of water extract of the Pleurotus Ferulae,that preliminarily extracted by ethanol,followed by removal of proteins,dialysis against water,precipitaion with alcohol and further purification by chromatography on DEAE-cellulose column and sephadex G-100 column.The thin layer chromatography indicated that polysaccharides from pleurotus ferulae were consisted of four monosaccharides,namely glucose,mannose,galactose and fructose.Basic on infrared and electrophoresis to analysis the mushroom ferulae polysaccharide fractions of structure,and it proved that Pleurotus ferulae polysaccharide was a kind of more pure polysaccharides.Finally,through the analysis by the AFM proved that Pleurotus ferulae polysaccharide molecule had highly branched structure,even between the sugar chain formed ring structure.