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2004•ACTA MICROBIOLOGICA SINICARequires access

Study on Soluble Polysaccharide by Acid Extraction from Pleurotus ferulae

Dong Kyun Hong

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Abstract

The water soluble polysaccharide PFA isolated by 3% trichloroacetic acid extraction from residues of fruit bodies of Pleurotus ferulae , Proteins were removed from PFA by Sevag method, and dialysed with water, precipitated with CTAB, and supernatant precipitated with alcohol, PF 3 was obtained. PF 3 contained 94 1% carbohydrate, the Mw of PF 3 was 1 975×10 6 The components and structure of PF 3 were analyzed by IR and GC. PF 2 consisted of rhamnose, xylose, glucose, galactose, mannose; and PF 3 contained α glucoside. It was found PF 3 had inhibition on RNase,inhibition rate was 44 4% at 5mg/mL concentration of PF 3.

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What this paper is about

The water soluble polysaccharide PFA isolated by 3% trichloroacetic acid extraction from residues of fruit bodies of Pleurotus ferulae , Proteins were removed from PFA by Sevag method, and dialysed with water, precipitated with CTAB, and supernatant precipitated with alcohol, PF 3 was obtained. PF 3 contained 94 1% carbohydrate, the Mw of PF 3 was 1 975×10 6 The components and structure of PF 3 were analyzed by IR and GC. PF 2 consisted of rhamnose, xylose, glucose, galactose, mannose; and PF 3 contained α glucoside. It was found PF 3 had inhibition on RNase,inhibition rate was 44 4% at 5mg/mL concentration of PF 3.

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Available abstract

The water soluble polysaccharide PFA isolated by 3% trichloroacetic acid extraction from residues of fruit bodies of Pleurotus ferulae , Proteins were removed from PFA by Sevag method, and dialysed with water, precipitated with CTAB, and supernatant precipitated with alcohol, PF 3 was obtained. PF 3 contained 94 1% carbohydrate, the Mw of PF 3 was 1 975×10 6 The components and structure of PF 3 were analyzed by IR and GC. PF 2 consisted of rhamnose, xylose, glucose, galactose, mannose; and PF 3 contained α glucoside. It was found PF 3 had inhibition on RNase,inhibition rate was 44 4% at 5mg/mL concentration of PF 3.

Key concepts: Trichloroacetic acid, Pleurotus, Chemistry, Rhamnose, Polysaccharide, Xylose, Chromatography, Extraction (chemistry)

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