2009Pharmaceutical Journal of Chinese People's Liberation ArmyRequires access

Study of the Quality Standard for Bushen Zhuanggu Keli

Li Han

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Abstract

Aim To establish a quality standard for Bushen Zhumtggu Keli.Methods Icariin of Bushen Zhuanggu Keli was determined by HPLC.Fructus Corni and Rhizoma Drynariae were identified by TLC.The determination was carried out by HPLC with an Agilent HC18(150mm×4.6mm,5μm)column.Acctonitrila and water(30:70) were used as a mobile phase with the detection wavelength at 270nm.Results Icariin showed a good linear relationship within the range of 0.096 2~0.673 4μg(r2=0.999 6).The average recovery of Icariin was 94.4%(RSD=1.8%,n=6).Conclusion The results are stable with good reproducibility.This method is reliable,which can be used for quality control of the capsules.

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What this paper is about

Aim To establish a quality standard for Bushen Zhumtggu Keli.Methods Icariin of Bushen Zhuanggu Keli was determined by HPLC.Fructus Corni and Rhizoma Drynariae were identified by TLC.The determination was carried out by HPLC with an Agilent HC18(150mm×4.6mm,5μm)column.Acctonitrila and water(30:70) were used as a mobile phase with the detection wavelength at 270nm.Results Icariin showed a good linear relationship within the range of 0.096 2~0.673 4μg(r2=0.999 6).The average recovery of Icariin was 94.4%(RSD=1.8%,n=6).Conclusion The results are stable with good reproducibility.This method is reliable,which can be used for quality control of the capsules.

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Available abstract

Aim To establish a quality standard for Bushen Zhumtggu Keli.Methods Icariin of Bushen Zhuanggu Keli was determined by HPLC.Fructus Corni and Rhizoma Drynariae were identified by TLC.The determination was carried out by HPLC with an Agilent HC18(150mm×4.6mm,5μm)column.Acctonitrila and water(30:70) were used as a mobile phase with the detection wavelength at 270nm.Results Icariin showed a good linear relationship within the range of 0.096 2~0.673 4μg(r2=0.999 6).The average recovery of Icariin was 94.4%(RSD=1.8%,n=6).Conclusion The results are stable with good reproducibility.This method is reliable,which can be used for quality control of the capsules.

Key concepts: Icariin, Quality standard, Chromatography, Reproducibility, High-performance liquid chromatography, Chemistry, Linear relationship, Linear range

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