Measurement of the content of Icariin in Bushen Zhuanggu Granula by HPLC

Objective: Bushen Zhuanggu Granula is a medicinal preparation made in Guangzhou General Hospital,with Herb Epimedii as the main ingredient.The aim of this study is to establish a method for measuring the content of Icariin in Bushen Zhuanggu Granula by high performance liquid chromatography(HPLC).Methods: Three batches of Bushen Zhuanggu Granula were measured for the content of Icariin by HPLC with an Agilent HC-18(150 mm × 4.6 mm,5 μm) column at the column temperature of 30 ℃,acetonitrile-water(30:70) as the mobile phase and the detection wavelength at 270 nm.Results: Icariin could be isolated from the other components,and showed a good linear relationship with the peak area at the range of 0.096 2-0.673 4 μg,r2=1(P0.001).The average recovery of Icariin was 94.4%(RSD = 1.83%,n=6).Conclusion: This method is sensitive,accurate,reproducible,specific and efficient for the measurement of the content of Bushen Zhuanggu Granula.