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2004•The Chinese Journal of Modern Applied PharmacyRequires access

Determination of icariin in Bushen Zhuanggu pills by HPLC

Xiaoni Li

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Abstract

OBJECTIVE To develop an HPLC method for the assay of icariin in Bushen Zhuanggu pill. METHOD The sample was extracted with 75% ethanol followed by purification with polyamide column. The assay quantitative analysis was performed on a C 18 column with a mobile phase of methanol-water-glacial acetic acid (55∶45∶1), and detection wavelength at 270 nm. The determination was according to the external standard method.RESULTS Good linearity of icariin was showed within the range of 0.084～1.008μg (r = 0.999 9). The average recovery was 99.4%, and RSD was 2.2%.CONCLUSION The method is simple and accurate. It is suitable for the quality control of this preparation.

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What this paper is about

OBJECTIVE To develop an HPLC method for the assay of icariin in Bushen Zhuanggu pill. METHOD The sample was extracted with 75% ethanol followed by purification with polyamide column. The assay quantitative analysis was performed on a C 18 column with a mobile phase of methanol-water-glacial acetic acid (55∶45∶1), and detection wavelength at 270 nm. The determination was according to the external standard method.RESULTS Good linearity of icariin was showed within the range of 0.084～1.008μg (r = 0.999 9). The average recovery was 99.4%, and RSD was 2.2%.CONCLUSION The method is simple and accurate. It is suitable for the quality control of this preparation.

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Available abstract

OBJECTIVE To develop an HPLC method for the assay of icariin in Bushen Zhuanggu pill. METHOD The sample was extracted with 75% ethanol followed by purification with polyamide column. The assay quantitative analysis was performed on a C 18 column with a mobile phase of methanol-water-glacial acetic acid (55∶45∶1), and detection wavelength at 270 nm. The determination was according to the external standard method.RESULTS Good linearity of icariin was showed within the range of 0.084～1.008μg (r = 0.999 9). The average recovery was 99.4%, and RSD was 2.2%.CONCLUSION The method is simple and accurate. It is suitable for the quality control of this preparation.

Key concepts: Icariin, Chromatography, Chemistry, High-performance liquid chromatography, Acetic acid, Medicine, Biochemistry, Alternative medicine

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