Study on Tissue Culture and Rapid Propagation Technology of Phalaenopsis amabilis(L.)C.L.Blume

Flower peduncles of Phalaenopsis amabilis(L.)C.L.Blume were cultured to induce seedlings on medium,then shoots were cultured as explants to produce shoot cluters in vitro.The effects of different plant growth regulators and their concentrations on the germination of dormant buds,induction of clusters buds and vitro rooting were studied to select the best medium.The effects of activated charcoal,PVP,temperature,dark culture on the medium browning were also investigated.The results showed that:the best media inducing dormant buds was 1/2 MS+6-BA 3.0 mg/L+NAA 0.2 mg/L+10% banana juice+3% sucrose+0.6% agar,the induction rate of shoots was up to 87.5%.The best media inducing clusters buds was 1/2 MS+6-BA 6.0 mg/L+NAA 0.1 mg/L+10% banana juice+3% sucrose+0.6% agar,the multiplication coefficient was up to 3.15.The best media inducing rooting was 1/2 MS+NAA 0.5 mg/L+1 000 mg/L activaed charcoal+3% sucrose+0.6% agar,the rooting rate reached to 95%,average number of roots was about 3.11 stripes/plant,transplant survival rate was 90% after hardening seedling.