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Tissue culture and rapid micropropagation of Phalaenopsis amabilis

Zhang QiXiang, Fang Yan-ming, Xiaoping Zhang

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Abstract

Flower peduncles of Phalaenopsis amabilis Bl. were cultured on MS_(0) medium to induce seedlings, then leaflets and shoots without apex of the plantlet were cultured as explants to produce protocorm-like bodies in vitro. The suitable media inducing protocorm-like bodies were MS + 3.0 mg·L~(-1) 6-BA + 0.5 mg·L~(-1) ZT + 30 mg·L~(-1) citric acid and MS + 5.0 mg·L~(-1) 6-BA + 30 mg·L~(-1) citric acid + 30% CM. Induction rate of shoots without apex was higher than that of leaves and it is up to 95%. The optimized medium for rooting was 1/4 MS+1.0 mg·L~(-1) 6-BA+0.1 mg·L~(-1) NAA, the rooting rate reached to 79%.

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What this paper is about

Flower peduncles of Phalaenopsis amabilis Bl. were cultured on MS_(0) medium to induce seedlings, then leaflets and shoots without apex of the plantlet were cultured as explants to produce protocorm-like bodies in vitro. The suitable media inducing protocorm-like bodies were MS + 3.0 mg·L~(-1) 6-BA + 0.5 mg·L~(-1) ZT + 30 mg·L~(-1) citric acid and MS + 5.0 mg·L~(-1) 6-BA + 30 mg·L~(-1) citric acid + 30% CM. Induction rate of shoots without apex was higher than that of leaves and it is up to 95%. The optimized medium for rooting was 1/4 MS+1.0 mg·L~(-1) 6-BA+0.1 mg·L~(-1) NAA, the rooting rate reached to 79%.

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Available abstract

Flower peduncles of Phalaenopsis amabilis Bl. were cultured on MS_(0) medium to induce seedlings, then leaflets and shoots without apex of the plantlet were cultured as explants to produce protocorm-like bodies in vitro. The suitable media inducing protocorm-like bodies were MS + 3.0 mg·L~(-1) 6-BA + 0.5 mg·L~(-1) ZT + 30 mg·L~(-1) citric acid and MS + 5.0 mg·L~(-1) 6-BA + 30 mg·L~(-1) citric acid + 30% CM. Induction rate of shoots without apex was higher than that of leaves and it is up to 95%. The optimized medium for rooting was 1/4 MS+1.0 mg·L~(-1) 6-BA+0.1 mg·L~(-1) NAA, the rooting rate reached to 79%.

Key concepts: Phalaenopsis, Plantlet, Citric acid, Explant culture, Micropropagation, Apex (geometry), Shoot, Botany

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