Establishment of technique system of rapid propagation through clustered buds pathway of Phalaenopsis

Phalaenopsis cultivar Dtps.Tailin Red Angel V31 was used as tissue culture material,investigations on the effect of disinfection time,basic medium,exogenous hormone on the induction of pedicel axillary bud,and effect of exogenous hormone on the propagation,rooting ability and vigorous condition of clustered buds were carried out.The results showed that when disinfected with 0.1% HgCl2 for 8 min,the induction rate of pedicel axillary bud were highest(85.19%).The induction rate of pedicel axillary bud were significantly higher on 1/2MS medium than MS medium.When the buds inoculated into 1/2MS+NAA 0.5mg/L+coconut juice 100ml/L+sucrose 30g/L+agar 7g/L with 5.0 mg/L 6-BA,the multiplication coefficient was highest(5.53);Adding coconut juice 100ml/L in 1/2MS+5.0 mg/L 6-BA+NAA 0.5mg/L+sucrose 30g/L+agar 7g/L,the multiplication coefficient was significantly higher than mashed potatoes and banana slurry.When the plantlets without roots inoculated into 1/2MS +mashed potatoes 80g/L+sucrose 20g/L+ agar 7g/L with 0.5 mg/L NAA,the numbers of rooting and leaf were significantly higher than other treatments,were 4.39 and 3.03;Adding mashed potatoes 80g/L in 1/2MS+NAA 0.5mg/L+sucrose 20g/L+agar 7g/L,the numbers of rooting and leaf were significantly higher than coconut juice and banana slurry.