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2004•Chinese Journal of Tropical AgricultureRequires access

Leaf Clonal Propagation of Phalaenopsis Blume

Xiyan Wang

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Abstract

Adventitious buds of Phalaenopsis Blume were induced directly from lea ves of plantlets of flower stalk culture without the development of protocorm-li ke body (PLB). Results showed that the MS + 6-BA 5 mg/L + NAA 1 mg/L + CW 100 mL/L produced an inducing rate of 32.8% adventitious buds and that the adventitiou s buds produced a rooting rate of 95% when cultured under the hyponex(7-6-19) 3 g/L + tryptone 1 g/L + activated charcoal 1 g/L + banana homogenate 100 g/L + IB A 1 mg/L + NAA 0.5 mg/L.

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What this paper is about

Adventitious buds of Phalaenopsis Blume were induced directly from lea ves of plantlets of flower stalk culture without the development of protocorm-li ke body (PLB). Results showed that the MS + 6-BA 5 mg/L + NAA 1 mg/L + CW 100 mL/L produced an inducing rate of 32.8% adventitious buds and that the adventitiou s buds produced a rooting rate of 95% when cultured under the hyponex(7-6-19) 3 g/L + tryptone 1 g/L + activated charcoal 1 g/L + banana homogenate 100 g/L + IB A 1 mg/L + NAA 0.5 mg/L.

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Available abstract

Adventitious buds of Phalaenopsis Blume were induced directly from lea ves of plantlets of flower stalk culture without the development of protocorm-li ke body (PLB). Results showed that the MS + 6-BA 5 mg/L + NAA 1 mg/L + CW 100 mL/L produced an inducing rate of 32.8% adventitious buds and that the adventitiou s buds produced a rooting rate of 95% when cultured under the hyponex(7-6-19) 3 g/L + tryptone 1 g/L + activated charcoal 1 g/L + banana homogenate 100 g/L + IB A 1 mg/L + NAA 0.5 mg/L.

Key concepts: Phalaenopsis, Activated charcoal, Stalk, Botany, Biology, Horticulture, Tissue culture, Chemistry

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