Relationship between Survivin protein and morphological changes of cervical cells,HPV infection and cervical intraepithelial neoplasia

Objective:To explore the relationship between Survivin protein and morphological changes of cervical cells,human papillomavirus(HPV) infection and cervical intraepithelial neoplasia(CIN).Methods:82 cases diagnosed as CINⅡ and CINⅢ definitely by histological examination in outpatient department of gynecology in the hospital from January 2008 to December 2009 were collected as study objects,and all the cases received liquid-based cytological test(LCT) of cervical exfoliative cells and HPV detection.Immunohistochemical method was used to detect Survivin protein level.Results:Survivin protein did not express in normal cervical tissues;Survivin protein expressed positively in cervical tissues of CINⅡ and CINⅢ,the positive rate was 100%,the expression level of Survivin protein in cervical tissues of CINⅢ was significantly higher than that in cervical tissues of CINⅡ(P0.05).The relationship between Survivin protein expression and morphological changes showed by LCT: the expression level of Survivin protein in high-grade squamous intraepithelial lesion(HSIL) group was significantly higher than that in low-grade squamous intraepithelial lesion(LSIL) group(P0.001);mild positive expression of Survivin protein was found in LSIL group;there was no significant difference in expression level of Survivin protein between atypical squamous cells of undetermined significance(ASCUS) group and negative for intraepithelial lesion or malignancy(NILM) group.Among 82 patients,76 patients were found with positive HPV,the main types of HPV infection were HPV-16,HPV-33 and HPV-58.In cervical tissues of CIN with single infection of HPV-16,Survivin protein showed strong positive and moderate positive expression;in cervical tissues of CIN with single infection of HPV-33,Survivin protein showed moderate positive expression;in cervical tissues of CIN with single infection of HPV-58,Survivin protein showed mild positive expression,there was significant difference among the three groups(P0.001).Conclusion:The positive rates of Survivin protein in cervical tissues of CINⅡ and CINⅢ are 100%,and the phenomenon indicates that Survivin protein is a sensitive immunohistochemical marker of CINⅡ and CINⅢ,which is related to the grades of CINⅡ and CINⅢ(P0.05).The expression of Survivin protein is correlated with the degrees of atypia of cervical epithelial cells.The difference of positive expression rate of Survivin protein among cervical tissues with different subtypes of HPV infection shows that there is a significant correlation between positive expression of Survivin protein and HPV-16 infection.