Experimental research on inhibitory effect of Yinlan Yigan Granules on reproduction of of HBV in vitro

[Objective] To explore the inhibitory effect of the serum containing pharmacodynamic ingredient of Yinlan Yigan Granule (strengthening the liver) on the secretion of HBsAg and HBeAg from the cell line of human liver carcinoma(HepG2.2.15) transinfected with gene of HBV. [Methods] The tissue culture and ELISA assay were used in the experiment. [Results] On the eighth day after treated with medicine-containing serum, the TC50 on HepG2.2.15 was 11.48%, the maximum nontoxic concentration of medicine-containing serum was 6.0%. The inhibitory rate of medicine-containing serum at concentration of 6.0%, 3.0% 1.5% and 0.75% in 2.2.15 cells on the 8th day was 7.89%, 5.8%, 1.6%, 1.6% respectively on HbsAg. All the suppression ratio on HBeAg was negative. [Conclusions] Yinlan Yigan Granules has light inhibitory effect on HBsAg-secreting Hep2.2.15 and no inhibitory effect on HbeAg is noted.