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The enzymatic hydrolysis of rapeseed proteinThe study on the hydrolysis conditions of flavorzyme

GU Wen-ying

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Abstract

Response surface methodology(RSM) was used to optimize the hydrolytic conditions.The optimal hydrolytic conditions for Flavorzyme were: 55.5℃， pH6.2， enzyme/substrate ratio 74.3 LAPU/g, substrate/solvent ratio 12％ (W/V), reaction time 3h. Under the optimum conditions,the relationship between the degree of hydrolysis(DH) and time was studied. The result proved that the predicative value was consistent with the test value.

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What this paper is about

Response surface methodology(RSM) was used to optimize the hydrolytic conditions.The optimal hydrolytic conditions for Flavorzyme were: 55.5℃， pH6.2， enzyme/substrate ratio 74.3 LAPU/g, substrate/solvent ratio 12％ (W/V), reaction time 3h. Under the optimum conditions,the relationship between the degree of hydrolysis(DH) and time was studied. The result proved that the predicative value was consistent with the test value.

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Available abstract

Response surface methodology(RSM) was used to optimize the hydrolytic conditions.The optimal hydrolytic conditions for Flavorzyme were: 55.5℃， pH6.2， enzyme/substrate ratio 74.3 LAPU/g, substrate/solvent ratio 12％ (W/V), reaction time 3h. Under the optimum conditions,the relationship between the degree of hydrolysis(DH) and time was studied. The result proved that the predicative value was consistent with the test value.

Key concepts: Hydrolysis, Rapeseed, Substrate (aquarium), Response surface methodology, Chemistry, Predicative expression, Enzymatic hydrolysis, Solvent

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