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2002•Journal of Zhengzhou Grain CollegeRequires access

APPLICATION OF RESPONSE SURFACE METHODOLOGY IN THE STUDY OF ENZYMATIC HYDROLYSIS OF RAPESEED PROTEIN

Xingfeng Guo

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Abstract

Response Surface Methodology was used to analyze the enzymatic hydrolysis conditions of rapeseed protein.The optimum conditions of flavorzyme hydrolysis were at 55℃ for 3hr,pH6.2,enzyme/substrate ratio 74.3 (LAPU/g),substrate/solvent ration 12%(w/v),the hydrolysis degree was 26.4%.The relationship between the degree of hydrolysis(DH)and hydrolysis time was also studied.The result proved that the predicative value was consistent with the test one.

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What this paper is about

Response Surface Methodology was used to analyze the enzymatic hydrolysis conditions of rapeseed protein.The optimum conditions of flavorzyme hydrolysis were at 55℃ for 3hr,pH6.2,enzyme/substrate ratio 74.3 (LAPU/g),substrate/solvent ration 12%(w/v),the hydrolysis degree was 26.4%.The relationship between the degree of hydrolysis(DH)and hydrolysis time was also studied.The result proved that the predicative value was consistent with the test one.

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Available abstract

Response Surface Methodology was used to analyze the enzymatic hydrolysis conditions of rapeseed protein.The optimum conditions of flavorzyme hydrolysis were at 55℃ for 3hr,pH6.2,enzyme/substrate ratio 74.3 (LAPU/g),substrate/solvent ration 12%(w/v),the hydrolysis degree was 26.4%.The relationship between the degree of hydrolysis(DH)and hydrolysis time was also studied.The result proved that the predicative value was consistent with the test one.

Key concepts: Hydrolysis, Rapeseed, Enzymatic hydrolysis, Response surface methodology, Substrate (aquarium), Chemistry, Enzyme, Chromatography

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