Preparation of florfenicol sodium succinate and its pharmacokinetics characteristics in rabbits

Florfenicol sodium succinate(Fl-SNa) was prepared using 4-dimethylaminopyridine(DMAP) as catalyst.The blood samples from the rabbits intramuscularly-injected with a single dose of either florfenicol solution or Fl-SNa solution were collected to measure florfenicol concentrations in plasma by high performance liquid chromatography(HPLC).The pharmacokinetic parameters were analyzed with DAS2.0 software.In results,Fl-SNa was successfully prepared.The major pharmacokinetic parameters of florfenicol and Fl-SNa were shown as Cmax of 8.863 mg/L±0.254 mg/L vs.7.363 mg/L±0.085 mg/L,tmax of 1.0 h vs.2.0 h,and AUC0-∞ of 28.851 mg/L·h±0.494 mg/L·h vs.39.187 mg/L·h±0.413 mg/L·h,respectively.In conclusion,HPLC was suitable for the determination of florfenicol concentration in plasma of rabbits,and the Fl-SNa could significantly increase bioavailability of florfenicol in rabbits.