Effects of Ultra-pulverization on Pharmacokinetics of Florfenicol in Chickens

Effects of ultra-pulverization on pharmacokinetics variance of florfenicol were studied in chickens.20 healthy broiler chickens were allocated into two groups with 10 chickens each.The chickens in groupⅠwere orally given single dose of florfenicol(no pulverization) at 30 mg/kg,the chickens in groupⅡwere orally given single dose of florfenicol ultrafine-powder at 30 mg/kg.The concentrations of florfenicol in plasma were determined by high performance liquid chromatography with detection limit of 0.004 μg/mL.The pharmacokinetic data were calculated by 3p97 PK program and fitted a one-compartment open model with weight by 1/C2.The main pharmacokinetics parameters of florfenicol(no pulverization) and florfenicol ultrafine-powder in chickens were as follows respectively,t1/2ka(28.89±4.51) and(23.49±4.48)min,t1/2ke(101.66±9.97) and(104.57±16.25) min,Tmax(72.86±7.15) and(62.23±4.78) min,Cmax(7.94±0.78) and(8.65±0.67)μg/mL,AUC(33.01±7.73) and(36.87±3.63) μg/(mL·h).The results showed that ultra-pulverization could influence the pharmacokinetic parameters of florfenicol in healthy chickens significantly.It can accelerate the speed of absorption,enhance the concentration in plasma and system bioavailability.