2014•Journal of Shandong Institute of Physical Education and SportsRequires access
Injury of exercise-induced renal ischemia-reperfusion and the protective effect of herba cistanches combined with vitamin C in rats
Zhou Jun-fe
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Abstract
Objective: To study the Herba Cistanches combined with vitamin C's protective effect and mechanism of movement in rats' ischemia-reperfusion injury. Methods: Randomly divide 96 7-week-old male Wistar rats into 6 groups: control group( group C,12),general training group( group M,12),overtraining group( group OM,24),cistanche + overtraining group( group COM,16),vitamin C + overtraining group( group VCOM,16),and cistanche + vitamin C + overtraining group( group CVCOM,16),take a 56-day of exhaustive swimming training. The COM group,CVCOM group with professional gavage for gastric lavage( ig),once a day,the dose of 6. 01 g. kg-1,Ig volume 5mL. kg-1; group VCOM,group CVCOM ip take intraperitoneal injection, 100 mg. kg-1,once a day,the injection volume is 0. 5 mL; other groups ig normal saline. Detect biochemical parameters of serum urea nitrogen and serum creatinine 24 hours later after the last training. Results: 1) In the 8 week of overtraining,rats resulted in movement of renal ischemia reperfusion,the ultrastructure of kidney was severely damaged,the histopathological changes obviously, function was seriously damaged. 2) Serum creatinine and blood urea nitrogen rise. OM group( P 0. 01),COM group( P 0. 01),VCOM group( P 0. 01),CVCOM group( P 0. 05) higher than in group C; COM group( P 0. 05),VCOM group( P 0. 05),CVCOM group( P 0. 01) lower than the OM group; COM group( P 0. 05),CVCOM groups( P 0. 01) lower than the VCOM group. 3) SOD activity decreased. OM group( P 0. 01),COM group( P 0. 01),of VCOM group( P 0. 01),CVCOM group( P 0. 05) lower than in group C; M group( P 0. 05), COM group( P 0. 05),VCOM group( P 0. 05),CVCOM group( P 0. 01) higher than the OM group; COM group( P 0. 05),CVCOM group( P 0. 01) higher than the VCOM group. 4) The content of MDA increased,OM group( P 0. 01),COM group( P 0. 01),VCOM group( P 0. 01),CVCOM group( P 0. 05) higher than in group C; COM group( P 0. 05),VCOM group( P 0. 05),CVCOM group( P 0. 01) lower than the OM group; COM group( P 0. 05),CVCOM groups( P 0. 01) lower than the VCOM group. Conclusion: 1) Overtraining can induce the ischemia-reperfusion in rats. 2) Cistanche deserticola Y. C. Ma and vitamin C can enhance the activity of SOD,scavenging oxygen free radicals,reduce lipid peroxidation,they have a protective effect on exercise-induced ischemia-reperfusion kidney. 3) Cistanche is better than vitamin C,but they worked even better when taken both.