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2009•Xibei zhiwu xuebaoRequires access

Establishment of in vitro regeneration system of poplar hybrid 717 and 353.

Bin Wang, Bailian Li, Jinfeng Zhang, Hong XiuLing, MU Chen-li

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Abstract

The regeneration of adventitious buds and the rooting of shoots from the leaves of hybrid 717 from Populus tremula×Populus alba and 353 from Populus tremula×Populus tremuloides with different genotypes and hormones combination were studied.The results showed that:the optimum medium for adventitious buds regeneration of hybrid 717 was MS+0.5 mg/L 6-BA+0.2 mg/L NAA;The optimum medium for adventitious buds regeneration of hybrid 353 was MS+0.5 mg/L 6-BA+0.2 mg/L NAA+0.01 mg/L TDZ;The optimum medium for rooting of shoots was 1/2 MS+0.2 mg/L IBA+0.2 mg/L NAA,with a rooting efficiency of 100%.The best leaves for adventitious buds regeneration were on the tip 1st～3rd leaf of stems.20 mg/L kanamycim could inhibit the bud regeneration of the hybrid 717 and 353,40 mg/L kanamycim could inhibit the root regeneration of the hybrid 717 and 353 from the shoots.

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What this paper is about

The regeneration of adventitious buds and the rooting of shoots from the leaves of hybrid 717 from Populus tremula×Populus alba and 353 from Populus tremula×Populus tremuloides with different genotypes and hormones combination were studied.The results showed that:the optimum medium for adventitious buds regeneration of hybrid 717 was MS+0.5 mg/L 6-BA+0.2 mg/L NAA;The optimum medium for adventitious buds regeneration of hybrid 353 was MS+0.5 mg/L 6-BA+0.2 mg/L NAA+0.01 mg/L TDZ;The optimum medium for rooting of shoots was 1/2 MS+0.2 mg/L IBA+0.2 mg/L NAA,with a rooting efficiency of 100%.The best leaves for adventitious buds regeneration were on the tip 1st～3rd leaf of stems.20 mg/L kanamycim could inhibit the bud regeneration of the hybrid 717 and 353,40 mg/L kanamycim could inhibit the root regeneration of the hybrid 717 and 353 from the shoots.

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Available abstract

The regeneration of adventitious buds and the rooting of shoots from the leaves of hybrid 717 from Populus tremula×Populus alba and 353 from Populus tremula×Populus tremuloides with different genotypes and hormones combination were studied.The results showed that:the optimum medium for adventitious buds regeneration of hybrid 717 was MS+0.5 mg/L 6-BA+0.2 mg/L NAA;The optimum medium for adventitious buds regeneration of hybrid 353 was MS+0.5 mg/L 6-BA+0.2 mg/L NAA+0.01 mg/L TDZ;The optimum medium for rooting of shoots was 1/2 MS+0.2 mg/L IBA+0.2 mg/L NAA,with a rooting efficiency of 100%.The best leaves for adventitious buds regeneration were on the tip 1st～3rd leaf of stems.20 mg/L kanamycim could inhibit the bud regeneration of the hybrid 717 and 353,40 mg/L kanamycim could inhibit the root regeneration of the hybrid 717 and 353 from the shoots.

Key concepts: Shoot, Regeneration (biology), Botany, Biology, Basal shoot, Murashige and Skoog medium, Horticulture, Tissue culture

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