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2014•Heilongjiang nongye kexueRequires access

Study on Tissue Culture and Rapid Propagation of Dendrobium candidum

Cao Tian-x

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Abstract

In order to establish the rapid propagation system of Dendrobium candidum,taking its stem as materials,the effects of medium types,hormone concentration on proliferation and rooting of Dendrobium candidum were studied.The results showed that the best sterilization method for explants was to treat by 75%ethyl alcohol for 30sand then 0.1% HgCl2for 6min.The optimum basic medium was MS.The greatest number of propagation and shoot forming were found in the MS medium supplemented with 1 mg·L-1 6-BA and 0.5mg·L-1 NAA.Roots were easily formed by the addition of IBA,and the treatment of 3.0mg·L-1 IBA in MS medium was more effective for growth of roots in vitro.The planting survival rate achieved 90.6%in simplex growing medium with moss,it was higher than the other growing media.

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What this paper is about

In order to establish the rapid propagation system of Dendrobium candidum,taking its stem as materials,the effects of medium types,hormone concentration on proliferation and rooting of Dendrobium candidum were studied.The results showed that the best sterilization method for explants was to treat by 75%ethyl alcohol for 30sand then 0.1% HgCl2for 6min.The optimum basic medium was MS.The greatest number of propagation and shoot forming were found in the MS medium supplemented with 1 mg·L-1 6-BA and 0.5mg·L-1 NAA.Roots were easily formed by the addition of IBA,and the treatment of 3.0mg·L-1 IBA in MS medium was more effective for growth of roots in vitro.The planting survival rate achieved 90.6%in simplex growing medium with moss,it was higher than the other growing media.

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Available abstract

In order to establish the rapid propagation system of Dendrobium candidum,taking its stem as materials,the effects of medium types,hormone concentration on proliferation and rooting of Dendrobium candidum were studied.The results showed that the best sterilization method for explants was to treat by 75%ethyl alcohol for 30sand then 0.1% HgCl2for 6min.The optimum basic medium was MS.The greatest number of propagation and shoot forming were found in the MS medium supplemented with 1 mg·L-1 6-BA and 0.5mg·L-1 NAA.Roots were easily formed by the addition of IBA,and the treatment of 3.0mg·L-1 IBA in MS medium was more effective for growth of roots in vitro.The planting survival rate achieved 90.6%in simplex growing medium with moss,it was higher than the other growing media.

Key concepts: Explant culture, Moss, Sterilization (economics), Dendrobium, Tissue culture, Botany, Shoot, Sowing

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