Dynamic Changes of Serum IL-2/IL-10 in Rats with Acute Necrotizing Pancreatitis and Intervention by Xingding

Objective To observe the dynamic changes of serum IL-2/IL-10 in acute necrotizing pancreatitis (ANP) in rats and to evaluate the therapeutic effectiveness of Xingding on IL-2/IL-10. Methods A total of 96 rats were randomly divided into three groups:(1) control; (2) ANP without treatment; (3) ANP treated with Xingding. The animals were sacrificed at 0.5,2,6 and 12 h after induction of pancreatitis. The blood samples of rats in each group were obtained via superior mesenteric vein to measure levels of IL-2 and IL-10. Meanwhile,pancreas were collected to observe pathological changes using a validated histology score. Results Comparison of control group,the pathological changes were aggravated significantly in ANP group. Levels of serum IL-2 were significantly higher than those at 0.5,2,6,12 h after establishment of the model (P 0.01 or P 0.05).Significantly increased the serum concentrations of IL-10 at 6 and 12 h in ANP group (P 0.01). The values of IL-2/IL-10 were higher significantly than those of control group at 2 h,and it reached peak at 6 h and returned to the control level at 12 h (P﹥ 0.05). Comparison of ANP group,the pathological changes were alleviated significantly in T group. Levels of serum IL-2 were significantly lower than those in ANP group at 6,12 and 24 h (P 0.01). Significantly decreased the serum concentrations of IL-10 at 12 and 24 h (P 0.05). The values of IL-2-α/IL-10 were decreased significantly than those of ANP group from 6 h. Conclusion The value of IL-2/IL-10 was shown on a trend which was turned from elevation to relative equilibrium and then into declining in ANP. Salviae miltiorrhizae can reduce the value of IL-2/IL-10.The mechanism we thought was that Xingdin could bidirectionally adjust TNF-α and IL-10.