Expression and significance of serum resolvin D1 in rats with acute necrotizing pancreatitis

Objective To observe the expression of serum resolvin D1(RvD1),IL-6 and IL-10in rats with acute necrotizing pancreatitis(ANP),and investigate the role of RvD1in nosogenesis of ANP.Methods Seventy SD rats were randomly divided into the ANP group(n=35)and the sham-operation group(n=35).In the ANP group,the rats were induced by the retrograde injection of bilio-pancreatic duct with 4% sodium taurocholate and sacrificed after taking the blood sample and the pancreas 3 h,6 h,12 h,24 h and 48 h after cessation of sodium taurocholate injection.The pathologic changes of pancreas were observed and graded under microscope,the level of serum amylase was detected with biochemical analyzer,and the level of serum RvD1,IL-6,IL-10 were evaluated using ELISA.Results The serum amylase level began to increase at 3 h after sodium taurocholate injection and reached the peak value at 6 h[(8244.00±2949.83)U/L],and were significantly higher than those of the sham-operation group all the time(P0.05).Pancreatic injuries of the ANP group aggravated with time,the pathologic score of 24 h was(11.91±1.312),and were significantly higher than those of the sham-operation group(P 0.05).The level of serum RvD1 in the ANP group increased gradually with time(3 h~24 h),reached the peak level at 24 h(39.48±15.28)pg/ml,was significantly higher than that of the sham-operation group(P0.05),and positively related to the level of serum IL-6(r=0.362,P0.05).The level of serum IL-6in the ANP group was significantly higher than that of the sham-operation group at 6 h,12 h and 24 h(P0.05),increased gradually with time,and reached the peak level at 24 h(1293.26±428.12)pg/mL.The level of serum IL-10 in the ANP group was significantly higher than that of the shamoperation group at 3 h,24 h,48 h(P0.05).Conclusion The level of serum resolvin might be related to the severity of acute pancreatitis(AP),and might play the role of anti-inflammatory in AP.