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Studies on tissue culture of Cerasus humilis Bunge

Mingwei Zhang

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Abstract

Experiment was carried out using three varieties of Cerasus humilis Bunge as material for studies of its tissue culture and rapid propagation techniques.The results showed that: ①The disinfection effects was the best using the lateral bud of young stems as explant.②The medium MS+6-BA 0.4 mg/L+ IAA 0.4 mg/L+30 g/L sugar+6 g/L agar was the optimal one for germination,and the medium MS+6-BA 0.4 mg/L+IAA 0.5 mg/L+30 g/L sugar+6 g/L agar was the optimal one for differentiation. ③The effective medium for rooting was 1/2MS+IBA 0.5 mg/L+30 g/L sugar+6 g/L agar. ④The best transplanting base material was mountain soil.

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What this paper is about

Experiment was carried out using three varieties of Cerasus humilis Bunge as material for studies of its tissue culture and rapid propagation techniques.The results showed that: ①The disinfection effects was the best using the lateral bud of young stems as explant.②The medium MS+6-BA 0.4 mg/L+ IAA 0.4 mg/L+30 g/L sugar+6 g/L agar was the optimal one for germination,and the medium MS+6-BA 0.4 mg/L+IAA 0.5 mg/L+30 g/L sugar+6 g/L agar was the optimal one for differentiation. ③The effective medium for rooting was 1/2MS+IBA 0.5 mg/L+30 g/L sugar+6 g/L agar. ④The best transplanting base material was mountain soil.

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Available abstract

Experiment was carried out using three varieties of Cerasus humilis Bunge as material for studies of its tissue culture and rapid propagation techniques.The results showed that: ①The disinfection effects was the best using the lateral bud of young stems as explant.②The medium MS+6-BA 0.4 mg/L+ IAA 0.4 mg/L+30 g/L sugar+6 g/L agar was the optimal one for germination,and the medium MS+6-BA 0.4 mg/L+IAA 0.5 mg/L+30 g/L sugar+6 g/L agar was the optimal one for differentiation. ③The effective medium for rooting was 1/2MS+IBA 0.5 mg/L+30 g/L sugar+6 g/L agar. ④The best transplanting base material was mountain soil.

Key concepts: Agar, Sugar, Transplanting, Explant culture, Botany, Tissue culture, Biology, Germination

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