Establishment of Rapid Propagation System of Cerasus humilis by Tissue Culture
Shuming Chen
Abstract
Shuming Chen
Abstract
Take 4 species of young stem of Cerasus humilis as explant,and 1/2MS,MS as culture medium.Rapid propagation system of Cerasus humilis by tissue culture was set up.The results showed that the optimal medium for primary culture was MS+0.1 mg/L NAA + 1.0mg/L 6-BA and MS+0.4 mg/L NAA + 1.0 mg/L 6-BA.The optimal medium for proliferation were MS+0.2mg/L NAA0.8mg/L6-BA and MS+ 0.2mg/LNAA+ 0.6mg/L6-BA+0.05mg/LPP333.And the best cullture medium has been defined respectively.
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Take 4 species of young stem of Cerasus humilis as explant,and 1/2MS,MS as culture medium.Rapid propagation system of Cerasus humilis by tissue culture was set up.The results showed that the optimal medium for primary culture was MS+0.1 mg/L NAA + 1.0mg/L 6-BA and MS+0.4 mg/L NAA + 1.0 mg/L 6-BA.The optimal medium for proliferation were MS+0.2mg/L NAA0.8mg/L6-BA and MS+ 0.2mg/LNAA+ 0.6mg/L6-BA+0.05mg/LPP333.And the best cullture medium has been defined respectively.
Key concepts: Explant culture, Tissue culture, Botany, Murashige and Skoog medium, Biology, Horticulture, In vitro, Biochemistry