Studies on tissue culture of Gaiguo 4,a line of Cerasus humilis

Experiment was carried out using the upper tender stem in Autumn and the inceptive tender stem in Spring of Gaiguo 4 (a line of Cerasus humilis Bunge) as material, and utilizing the orthogonal test to obtained the optimal medium for its tissue culture. Result showed: the inceptive tender stem in spring was the best explant. The best method for disinfectant was 70% to 75% alcohol (washed for 10s to 30s) and 0.1% mercuric chloride (washed for 6 min). The optimal medium for inducing shoot was improved MS + NAA 0.1mg/L + 6-BA 1.0 mg/L+ 20 g/L sugar + 7 g/L agar; the optimal medium for shoot generation was improved MS + 6-BA 2.0 mg/L+NAA 0.02 mg/L+GA3 0.2 mg/L+20 g/L sugar+ 7 g/L agar;the optimal medium for rooting is 1/2MS + IBA 1.0 mg/L + KT 0.02 mg/L + 20 g/L sugar + 7 g/L agar.