Application of multi-tumor marker protein chip in the diagnosis of ovarian cancer

Objective:To investigate the application of multi-tumor marker protein chip in the diagnosis of ovarian cancer. Methods:The serum levels of 12 tumor markers including CA125,CA19-9,CA153,CA242,CEA, AFP, β-HCG, NSE, PSA, f-PSA, ferritin and HGH were determined by using multi-tumor marker protein chip in 66 patients with malignant ovarian cancer,64 patients with benign gynecologic diseases and 144 healthy women,and its diagnostic value was evaluated. Results:The levels of CA19-9, CEA, CA242, ferritin, AFP, CA125 and CA153 in patients with ovarian cancer were significantly higher than those in patients with benign diseases and healthy controls (P0.01). The positive rates of CA125, ferritin, CA19-9, CEA, CA242, CA153 were 68.2%, 31.8%, 30.3%, 22.7%, 19.7% and 19.7% in patients with ovarian cancer, respectively, which were significantly higher than those in benign diseases and healthy controls (P0.001). The protein chip had significantly higher positive rate than any single item of the 12 tumor markers (P0.001). The diagnostic sensitivity and accuracy of the combined detection of CA125+CA19-9 and CA125+CA19-9+CEA were much higher than those of single CA125 for ovarian cancer. The sensitivity, specificity, accuracy, positive and negative predictive values by protein chip detection were 87.9%, 93.3%, 92.0%, 80.6% and 96.0%,respectively. As compared with CA125 alone, the diagnostic sensitivity and accuracy of the protein chip increased from 68.2% and 90.5% to 87.9% and 92.0%, respectively. Conclusion:The combined detection of tumor markers by protein chip technique is helpful in improving the diagnostic sensitivity and accuracy and valuable for the diagnosis of ovarian cancer.