2005•Biaoji mianyi fenxi yu linchuangRequires access
Protein biochip technology detecting multi-tumor markers and their clinical application values in three kinds of carcinomas
Yong Liu, Li Fuquan, Jiajia Wang, Su Chen, Cheng Xixiang
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Abstract
To study the clinical application value of quantitative detection of 12 tumor markers in hepatocellular carcinoma, pancreatic cancer and ovarian cancer, the 12 tumor markers—CA125, ferritin, CEA, AFP, CA19-9,CA15-3, NSE, CA242, PSA, f-PSA, HGH and β-HCG in the serum of 48 patients with hepatocellular carcinoma, 29 patients with pancreatic cancer, 42 patients with ovarian cancer, 74 patients with benign disease and 66 healthy control persons were quantitatively detected by the protein biochip determination system of C-12. The results showed that ①The levels of AFP, CA125, ferritin, CEA, CA19-9, CA242 and β-HCG in patients with hepatocellular carcinoma were significantly higher than those in patients with benign disease and controls. The sensitivity, specificity, positive and negative predictive values, diagnostic efficiency by combinative detection were 89.58%, 87.32%,70.49%,96.12% and 87.89%, respectively. ②The levels of CA19-9, CA125, CA242, ferritin and CEA in patients with pancreatic cancer were significantly higher than those in patients with benign disease and controls(P0.01). The sensitivity, specificity, positive and negative predictive values, diagnostic efficiency by combinative detection were 82.76%, 87.32%, 57.14%, 96.125% and 86.55%, respectively. ③The levels of CA125, CEA, CA15-3 and CA242 in patients with ovarian cancer were significantly higher than those in patients with benign disease and controls(P0.01). The CA19-9 level was significantly higher than that in healthy persons(P0.05). The sensitivity, specificity, positive and negative predictive values, diagnosing efficiency by combinative detection were 80.95%, 87.32%, 65.38%, 93.94% and 85.87%, respectively. In conclusion, combinative detection of various kinds of tumor markers should be adopted in diagnosis of different types of cancer. Detection of multi-tumor markers by using protein chip can be applied clinically for early screening diagnosis of tumor, as well as the patient’s prognosis and therapeutic effect.