Cloning and identification of mouse C3d cDNA

Objective To clone and identify cDNA of mouse C3d. Methods Total cell RNA was extracted from the liver tissue of mouse and the cDNA of C3d was amplified by reverse transcription polymerase chain reaction (RT PCR). The cDNA fragments were direc tly inserted into p MD 18 T plasmid. The recombinant C3d pMD 18 T was identified by restriction endonu cleases digestion and sequencing. Re sults The recombinant plasm id contain 1.0 kb insert fragment and the sequence of the insert was identical t o the published sequence encoding mouse C3d. Conclusion T he mouse C3d cDNA was successfully cloned.