CLONING AND IDENTIFICATION OF AA CHICKENS C3D CDNA

To clone and identify cDNA of AA chickens C3d,we extracted total cell RNA from the liver tissue of AA chickens and the cDNA of C3d was amplified by reverse transcription polymerase chain reaction(RT-PCR).The cDNA fragments were directly inserted into pMD18-T plasmid.The recombinant C3d-pMD18-T was identified by means of restriction endonu cleases digestion and sequence.The recombinant plasmid contains about 1.0 kb insert fragment and the sequence of the insert was identical to the published sequence encoding LaiHang chickens C3d.So the AA chicken C3d cDNA was successfully cloned.