Cloning and Sequence Analysis of Mouse Interleukin-33 Gene

Objective:To clone and analyze the cDNA sequence encoding mouse interleukin-33 (IL-33) gene.Methods:Total RNA was prepared from the spinal cord of BALB/c mouse,cDNA fragment encoding IL-33 gene was amplified by RT-PCR using specific primers,and then was cloned into pcDNA3.1 vector.Recombinant eukaryotic expression vector pcDNA3,1-mlL-33 was constructed and was transformed into the host E.coli strain TOP10 for identification.Inserted IL-33 gene was sequenced.Results:Sequence analysis indi- cated that the cDNA of IL-33 had the whole length of 801 bp with a complete open reading frame,which encoded the product of 266 amino acids.The cDNA shared 100% homology with the sequence of mRNA for mouse IL-33 gene in GeneBank.Conclusion:The whole-length cDNA of mouse IL-33 was successfully cloned,which laid a foundation for further studying on its biological function.