Expression and significance of a disintegrin and metalloproteinase 12 in placenta of preeclampsia

Objective To study the mRNA and protein expressions of the ADAM12 in placenta of preeclampsia patients and their roles on pathogenesis of preeclampsia.Methods Immunohistochemistry and in-situ hybridzation were used to investigate the expression of ADAM protein,ADAM12-S mRNA,and ADAM12-L mRNA in placenta of 31 women with preeclampsia(including 15 cases of mild preeclampsia,16 cases of severe preeclampsia) and 20 cases normal full term pregnant women,respectively.Results The protein expressions of ADAM12 were significantly increased in placenta of preeclampsia patients(P0.05);but there was no significant difference between severe and mild preeclampsia groups(P0.05). The expression of ADAM12-L mRNA were significantly increased in placenta of preeclampsia patients(P0.05);and there was also no significant difference between severe and mild preeclampsia groups(P0.05).The expressions of ADAM12-S mRNA were significantly increased in placenta of preeclampsia patients(P0.01);and there were significantly difference between severe and mild preeclampsia groups(P0.05).Conclusion The high expression of ADAM12 plays a crucial role in the pathogenesis of preeclampsia,and ADAM12-S directly connects with the level of preeclampsia.