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HPLC-DAD determination of oleanolic acid and ursolic acid in the different parts of Polygonum perfoliatum

Zhengping Wu

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Abstract

Objective:To establish a HPLC-DAD method for the determination of oleanolic acid and ursolic acid in the different parts of Polygonum perfoliatum L.Methods:Kromasil C18 column(4.6 mm×250 mm,5 μm)was adopted.The mobile phase consisted of methanol-0.5% phosphoric acid aqueous solution(10∶90)at a flow rate of 0.8 mL·min-1,and the column temperature was set at 30 ℃.The detection wavelength was 210 nm.Results:Oleanolic acid showed good relationship at the range of 0.228-2.850 μg(r=0.9998)and the recovery was 97.4% with 1.5%(n=6)of RSD.Ursolic acid showed good relationship at the range of 0.544-6.800 μg(r=0.9998)and the recovery was 96.9% with 1.2%(n=6)of RSD.Conclusion:The proposed method is accurate,simple,and promises to be applicable for the determination of oleanolic acid and ursolic acid in the different parts of P.perfoliatum L.

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Objective:To establish a HPLC-DAD method for the determination of oleanolic acid and ursolic acid in the different parts of Polygonum perfoliatum L.Methods:Kromasil C18 column(4.6 mm×250 mm,5 μm)was adopted.The mobile phase consisted of methanol-0.5% phosphoric acid aqueous solution(10∶90)at a flow rate of 0.8 mL·min-1,and the column temperature was set at 30 ℃.The detection wavelength was 210 nm.Results:Oleanolic acid showed good relationship at the range of 0.228-2.850 μg(r=0.9998)and the recovery was 97.4% with 1.5%(n=6)of RSD.Ursolic acid showed good relationship at the range of 0.544-6.800 μg(r=0.9998)and the recovery was 96.9% with 1.2%(n=6)of RSD.Conclusion:The proposed method is accurate,simple,and promises to be applicable for the determination of oleanolic acid and ursolic acid in the different parts of P.perfoliatum L.

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Available abstract

Objective:To establish a HPLC-DAD method for the determination of oleanolic acid and ursolic acid in the different parts of Polygonum perfoliatum L.Methods:Kromasil C18 column(4.6 mm×250 mm,5 μm)was adopted.The mobile phase consisted of methanol-0.5% phosphoric acid aqueous solution(10∶90)at a flow rate of 0.8 mL·min-1,and the column temperature was set at 30 ℃.The detection wavelength was 210 nm.Results:Oleanolic acid showed good relationship at the range of 0.228-2.850 μg(r=0.9998)and the recovery was 97.4% with 1.5%(n=6)of RSD.Ursolic acid showed good relationship at the range of 0.544-6.800 μg(r=0.9998)and the recovery was 96.9% with 1.2%(n=6)of RSD.Conclusion:The proposed method is accurate,simple,and promises to be applicable for the determination of oleanolic acid and ursolic acid in the different parts of P.perfoliatum L.

Key concepts: Ursolic acid, Oleanolic acid, Chemistry, Phosphoric acid, Chromatography, High-performance liquid chromatography, Methanol, Organic chemistry

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HPLC-DAD determination of oleanolic acid and ursolic acid in the different parts of Polygonum perfoliatum — Research Paper | ScholarLens