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2011•Journal of Henan UniversityRequires access

Determination of icariin in gushukang granula by HPLC

Qingqing Zhang

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Abstract

Objective:To establish a method for the determination of icariin in gushukang granula by HPLC.Methods:The chromatographic conditions were:ODS-C18 column as analytic column,methanol-water-triethylamine(60:40:0.2),pH 6.0 adjusted by acetic acid as mobile phase,the flow rate was 1.0 mL/min,and the detection wave-length was set at 270 nm.Results:The linearity of icariin in the rang of 10.0 ～ 50.0 μg/mL was good.The average recovery was 99.8%,RSD=0.60%(n=6).Conclusion:This method is accurate and stabile,and it can be used for the quantity control of gushukang granula.

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What this paper is about

Objective:To establish a method for the determination of icariin in gushukang granula by HPLC.Methods:The chromatographic conditions were:ODS-C18 column as analytic column,methanol-water-triethylamine(60:40:0.2),pH 6.0 adjusted by acetic acid as mobile phase,the flow rate was 1.0 mL/min,and the detection wave-length was set at 270 nm.Results:The linearity of icariin in the rang of 10.0 ～ 50.0 μg/mL was good.The average recovery was 99.8%,RSD=0.60%(n=6).Conclusion:This method is accurate and stabile,and it can be used for the quantity control of gushukang granula.

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Available abstract

Objective:To establish a method for the determination of icariin in gushukang granula by HPLC.Methods:The chromatographic conditions were:ODS-C18 column as analytic column,methanol-water-triethylamine(60:40:0.2),pH 6.0 adjusted by acetic acid as mobile phase,the flow rate was 1.0 mL/min,and the detection wave-length was set at 270 nm.Results:The linearity of icariin in the rang of 10.0 ～ 50.0 μg/mL was good.The average recovery was 99.8%,RSD=0.60%(n=6).Conclusion:This method is accurate and stabile,and it can be used for the quantity control of gushukang granula.

Key concepts: Icariin, Chromatography, Triethylamine, High-performance liquid chromatography, Chemistry, Acetic acid, Medicine, Organic chemistry

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