Simultaneous Determination of Icariin and TanshinoneII_A in Fufang Xianlingpi Granula by High Performance Liquid Chromatography

Objective To establish a method for determining icariin and tanshinoneⅡA in Fufang Xianlingpi Granula.Methods High performance liquid chromatography was used for the content of icariin and tanshinoneⅡAwith linear gradient elution.The chromatography column was Kromasil C18(200 mm× 4.6 mm,0.45 μm);the mobile phase was acetonitrile-water;the flow rate was 1.0 ml/min;the detective wavelength was 270 nm,and the column temperature was 25℃.Results The linear range of icariin was 7.51-375.50 μg/ml(r=0.9997).The average recovery of icariin was 96.27% and RSD was 2.74%(n=6).The linear range of tanshinone ⅡAwas 0.88-35.04 μg/ml(r=0.9999).The average recovery of tanshinoneⅡAwas 102.5%and RSD was 2.87%(n=6).Conclusion The method is simple,specific and reproducible,which can be used to determine icariin and tanshinoneⅡAin Fufang Xianlingpi Granula.