Expression of transforming growth factor-beta_1 and matrix metalloproteinase 9 mRNA and their significance in placenta of pregnant women with preeclampsia.

Objective:To study the mRNA expression of transforming growth factor-beta_1(TGF-β_1)and matrix metalloproteinase 9(MMP-9) in placental tissue of pregnant women with preeclampsia and their relation to the pathogenic causes of preeclampsia.Methods: Semi-quantitative RT-PCR analysis was used to investicate the TGF-β_1、MMP-9 mRNA expression levels in placental tissue of 10 cases of normal term pregnant women and 25 cases of women with preeclmpsia(11 cases of mild preeclampsia and 14 cases of severe preeclampsia).Results: Compared with the normal group,the mRNA expression level of TGF-β_1 of severe preeclampsia group was higher(P0.05),the mRNA expression level of MMP-9 of severe preeclampsia group was significantly lower(P0.01),there was no significance between the mild preeclampsia group and the normal group of the expression of TGF-β_1 and MMP-9.Conclusions:The expression of TGF-β_1 and MMP-9 was already changed at the level of transcription.By decreasing the expression of MMP-9,TGF-β_1 inhibited the shallow invasion of trophoblast,these invasion related genes maybe play an important role in the pathogenic causes of preeclampsia.