Expression of matrix metalloproteinase 9 and tissue inhibitor of metalloproteinase 1 mRNA in the placenta of preeclampsia.

Objective: quantitatively determining MMP9mRNA、TIMP1mRNA in the placenta tissue by reverse transcription -polymerase chain reaction (RT-PCR) in order to evaluate the role of MMP9 in preeclamosia. Methods: Study group was consisted of 22 women with preeclampsia (severe 14,slight 8),and the control group was consisted of 10 normal pregnancies. The expression of MMP9mRNA、TIMP1mRNA in the placenta tissue was detected by RT-PCR,and their quantities were noted by the density of MMP9/β-actin or TIMP1/β-actin. Results: The expression level of MMP9 of severe preeclampsia group was significant lower (0.05±0.04)than the control group and slight preeclampsia group. And the ratio of MMP9 and TIMP1 was significant lower(0.04±0.03). Conclusion:The results suggested that the expression of MMP9 was already reduced at the level of transcription, and increased expression of TIMP1 mRNA inhibited its activity furthmore.MMP9 has the importance in the forming and development of preeclamosia.