Embryogenic Callus and Plant Regeneration in Maize Inbred Lines GS01
Dan Yao
Abstract
Dan Yao
Abstract
In this experiment,used maize inbred line GS01 immature embryos as explants to induce callus,different media,sucrose concentration and 2,4-D concentrations were determined.Embryogenic callus produced after subculture,differentiation,rooting and transplanting to growth for the complete plant.Experiment show that maize inbred line GS01 in the N6 medium,2,4-D concentration of 1 mg/L,sucrose concentration of 30 g/L,the rate of embryogenic callus induction was high.
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In this experiment,used maize inbred line GS01 immature embryos as explants to induce callus,different media,sucrose concentration and 2,4-D concentrations were determined.Embryogenic callus produced after subculture,differentiation,rooting and transplanting to growth for the complete plant.Experiment show that maize inbred line GS01 in the N6 medium,2,4-D concentration of 1 mg/L,sucrose concentration of 30 g/L,the rate of embryogenic callus induction was high.
Key concepts: Callus, Subculture (biology), Sucrose, Inbred strain, Biology, Regeneration (biology), Explant culture, Botany