Establishment of Plant Regeneration System from Immature Embryos of Maize Inbred Lines Zheng58 and Dan340

Callus was induced from immature embryos of 9 maize inbred lines on MB medium and N6 medium.The effects of different genotypes,different mediums,explants sizes and different concentrations of phytohormone on the callus induction of immature embryos were studied.Differences in callus growth state from different genotypes were also analyzed.Regeneration system was successfully established via the callus pathway.The results showed that genotypes have significant effects on callus induction.Both genotype and medium affected the frequency of embryogenic callus formation.The choice size was 1.5-2.0 mm young embryos as explants to be advantageous to obtain perfect callus.2,4-dichlorophenoxyacetic acid(2,4-D)was necessary in callus induction,and the optimal concentration for embryogenic callus formation was 2.0-2.5 mg/L.