KiSS-1 and its receptor gene expression in breast cancer and the relationship to clinical significance

Objective To investigate the exprssion of KiSS-1 and hOT7T175 gene in breast cancer and its clinical significance.Methods The mRNA expression of KiSS-1 and hOT7T175 gene was detected in benign and malignant breast tissues by RT-PCR in 40 patients.Results In 40 cases of breast cancer tissues,the positive rate of KiSS-1 gene and hOT7T175 gene expression was 42.5% and 25% respectively,while in normal tissue the rate was 72.5% and 50% respectively.Loss of KiSS-1 and hOT7T175 gene expression in breast cancer was not correlated with clinical stage,tumor size.The positive KiSS-1 rate was 63.6% in those without lymph node metastasis,but only 16.7% in those with metastasis (P 0.01).Conclusion Loss of KiSS-1 gene expression may be a useful marker for detection of lymph node metastasis in breast cancer.