Expression of Kiss-1 gene in breast tumor tissues and its clinical significance

Objective To evaluate the expression of Kiss-1 mRNA in normal breast tissues and breast tumor tissues and its clinical significance.Methods The expression of Kiss-1 mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) in normal breast tissues and breast tumor tissues of 70 patients with breast cancer and 40 patients with fibroadenoma.Results The expression of Kiss-1 mRNA in breast cancer tissues (0.952±0.576) and fibroadenoma tissues (0.587±0.496) were higher than that in adjacent normal tissues(t=7.917,P=0.00;t=2.656,P=0.011).The expression of Kiss-1 mRNA in breast cancer tissues was higher than that in fibroadenoma tissues (t=3.365,P=0.001).The expression of Kiss-1 mRNA in breast cancer patients with lymph node metastasis (0.662±0.530),was lower than that in patients without lymph node metastasis (1.124±0.537) (t=3.495,P=0.001).Conclusion With the relatively high expression in breast tumor tissues,Kiss-1 mRNA may serve as a new marker of breast tumor.The low expression of Kiss-1 mRNA may be related to lymph node metastasis of breast cancer.