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Determination of Puerarin in Geqi Granule by HPLC

Lina Gong

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Abstract

Puerarin content was determined by high performance liquid chromatography(HPLC) method in Geqi granule.The Shim-pak clc-ODS C18(4.6 mm×150 mm,5 μm) pillar was selected with methanol-0.25 mol·L-1 phosphoric acid(10∶40) as the mobile phase,and the detection wavelength was 250 nm.Results showed that the calibration curve was linear within the range of 0.038~0.19 μg for puerarin.The average recovey was 97.4%(n=5) and the RSD was 1.72%.The method can be used for the quality control of Geqi granule owing to its good stability and repeatability.

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What this paper is about

Puerarin content was determined by high performance liquid chromatography(HPLC) method in Geqi granule.The Shim-pak clc-ODS C18(4.6 mm×150 mm,5 μm) pillar was selected with methanol-0.25 mol·L-1 phosphoric acid(10∶40) as the mobile phase,and the detection wavelength was 250 nm.Results showed that the calibration curve was linear within the range of 0.038~0.19 μg for puerarin.The average recovey was 97.4%(n=5) and the RSD was 1.72%.The method can be used for the quality control of Geqi granule owing to its good stability and repeatability.

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Available abstract

Puerarin content was determined by high performance liquid chromatography(HPLC) method in Geqi granule.The Shim-pak clc-ODS C18(4.6 mm×150 mm,5 μm) pillar was selected with methanol-0.25 mol·L-1 phosphoric acid(10∶40) as the mobile phase,and the detection wavelength was 250 nm.Results showed that the calibration curve was linear within the range of 0.038~0.19 μg for puerarin.The average recovey was 97.4%(n=5) and the RSD was 1.72%.The method can be used for the quality control of Geqi granule owing to its good stability and repeatability.

Key concepts: Chemistry, Puerarin, Chromatography, High-performance liquid chromatography, Repeatability, Granule (geology), Calibration curve, Phosphoric acid

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