2006•Lishizhen Medicine and Materia Medica ResearchRequires access

Determination of Puerarin in Jiangzhijianfei Granule by HPLC

Shao-Hua Lin, HE Yu-fang, Nan Min-lun, Wang Tie-zhu, Zhao Quan-cheng

Abstract

ObjectiveTo establish the HPLC method for the determination of puerarin in Jiangzhijianfei granule.MethodsThe chromatographic conditions included:The column is Shim-pack ODS C_(18) column(150 mm×4.6 mm,5 μm),the mobile phase is methol-water H_3PO_4,the detection wavelength is 250 nm and the column temperature is room temperature.ResultsThe linear range of puerarin was 0.114 0～0.570 0 μg,r=0.999 6.The average recovery of puerarin was 98.7% with RSD 0.67%.ConclusionThe mothod is accurate and can be used as a determination of puerarin in Jiangzhijianfei granule.

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Available abstract

Key concepts: Puerarin, Chromatography, Granule (geology), High-performance liquid chromatography, Chemistry, Linear range, Materials science, Detection limit