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2010•Pharmaceutical Journal of Chinese People's Liberation ArmyRequires access

Reversed-phase High Performance Liquid Chromatography Determination of Puerarin in Gehuang Granule

You Guang-fu

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Abstract

Objective To establish an high performance liquid chromatography method for determination of puerarin in Gehuang granule.Methods Shimpack VP-ODS(4.6 mm×250 mm,5 μm)was used with acetonitrile-water-acetic acid glacial(14∶85∶1) as the mobile phase.The flow rate was 1.0 ml/min,detected at 254 nm and room temperature.The external standard method was adopted for the assay.Results The injection quantity of Puerarin presented a good linear range of 0.0226-0.226 μg(r=0.9998).The average recovery was 100.35%,RSD=1.01%(n=6).Conclusion The method is good in separability,reproducibility and sensitivity.

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Objective To establish an high performance liquid chromatography method for determination of puerarin in Gehuang granule.Methods Shimpack VP-ODS(4.6 mm×250 mm,5 μm)was used with acetonitrile-water-acetic acid glacial(14∶85∶1) as the mobile phase.The flow rate was 1.0 ml/min,detected at 254 nm and room temperature.The external standard method was adopted for the assay.Results The injection quantity of Puerarin presented a good linear range of 0.0226-0.226 μg(r=0.9998).The average recovery was 100.35%,RSD=1.01%(n=6).Conclusion The method is good in separability,reproducibility and sensitivity.

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Available abstract

Objective To establish an high performance liquid chromatography method for determination of puerarin in Gehuang granule.Methods Shimpack VP-ODS(4.6 mm×250 mm,5 μm)was used with acetonitrile-water-acetic acid glacial(14∶85∶1) as the mobile phase.The flow rate was 1.0 ml/min,detected at 254 nm and room temperature.The external standard method was adopted for the assay.Results The injection quantity of Puerarin presented a good linear range of 0.0226-0.226 μg(r=0.9998).The average recovery was 100.35%,RSD=1.01%(n=6).Conclusion The method is good in separability,reproducibility and sensitivity.

Key concepts: Puerarin, Chromatography, Reproducibility, Granule (geology), Acetic acid, High-performance liquid chromatography, Chemistry, Acetonitrile

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