2000•Xibei zhiwu xuebaoRequires access
RFLP analysis of mtDNAs among three types of cytoplasmic male sterility and their F_(1) hybrids in wheat
Fang Yao, Sun Lanzhen, Chuanyou Li, Baoshen Liu, Gao Qingrong
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Abstract
T ,K and V type wheat cms lines,of which the cytoplasms derived from T.timopheevii,Ae.kotschyi and Ae.ventricosa ,respectively,were compared with their common maintainer,K and V type restorer,T type restorer,and their F 1 hybrids with mitochondrial DNA (mtDNA) RFLP analysis.The results showed that:(1) difference were found between not only the K ,V and T type cms lines,but also cms lines and their restorers,so mtDNA may be associated with cms in wheat;(2) polymorphism was found between T ,K type cms lines and their F 1 hybrids in ATP6/Hind Ⅲ,ATP6/BamHⅠ, 26S/Hind Ⅲ,26S/BamHⅠ combinations,which revealed that nuclear genes influence the structure of mtDNA of cms lines;(3) “sterility fertility restoration” is an ideal model system to study the mechanism of cms.Comparative on difference of mtDNAs between cms line and its F 1 hybrid was emphasized in our study.