Mitochondrial DNAs of Cytoplsamic Male Sterile Lines of K-and V-Type in Wheat

The mitochondrial DNAs(mtDNAs) of cytoplasmic male sterile(cms) lines of K- and V-type with thesame nuclear background were comparedwith that of their maintainer line byRFLP and RAPD analyses. Distinctpolymorphisms were identified among themtDNAs of cms-K, cms-V and theirmaintainer line (Figs. 1 ~ 4; Table 1),the orgniation and structure of mitochondrial genes such as atp9, atpA,coxII and cob were different remarkably among the hoe lines (Figs. 1 ~ 3;Table 1 ), which means that mtDNAsmay be responsible for the formation ofcms in wheat. However, it is difficult toidentify which mtDNA fragment is definitely responsible for becuse thereare so many differences between themtDNAs of line and its maintainerline. Both the formation of and thefertility restoration are the results of nucleo-cytoplasmic interaction, cms - fertility restorationis an ideal model systemto stuty the mechanism of cms. Comparing the structural as well as the restoration of differences of mtDNAs betweencms line and its FI hybrid with therestoration line will advance the identifyof cms-inducing factors.