RAPD Analysing of CMS-T, K, V mtDNAs and Cloning of mtDNA Fragments Associated with CMS K in Wheat

Mitochondrial DNAs(mtDNAs) from three types of cytoplasmic male sterility lines (CMS K, V and T) in wheat, of which the cytoplasm derived from Ae. kotschyi , Ae. ventricosa and T. timopheevii, respectively, were compared among CMSs and their maintainer, restorer and hybrids of CMS K and V. The results indicated that: (1) the mtDNAs from CMS K, V and T were different from each other and from their maintainer, this provided some evidence at molecular level for three kinds of CMS lines with different cytoplasms; (2) the genetic dendrogram was made and the relative genetic distance among 7 different wheat materials was calculated by cluster analysis with amplification products from 23 primers; (3) two specific fragments of mtDNA were then cloned into plasmid vector and converted into RFLP probes.Southern hybridization patterns revealed polymorphism between sterile line and maintainer line .